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KMID : 0379520030190010033
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한국독성학회지
2003 Volume.19 No. 1 p.33 ~ p.37
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Anti-inflammatory Effects of Asiaticoside on Inducible Nitric Oxide Synthase and Cyclooxygenase-2 in RAW 264.7 Cell Line
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Jew Sang-Sup
Bae Ok-Nam
Chung Jin-Ho
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Abstract
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Asiaticoside has been tested for the ability as an anti-inflammatory drug using lipopolysaccharide (LPS)-stimulated macrophage cell line (RAW 264.7 cell). LPS treatment induced dramatically inducible nitric oxide synthase (iNOS) in RAW cells. However, asiaticoside inhibited LPS-stimulated iNOS induction in a concentration-dependent manner. Especially, higher concentrations (＞50 mutextrm{M}) of asiaticoside completely blocked iNOS induction. In addition, LPS-stimulated expression of inducible cyclooxygenase (COX-2) and interleukin-1 alpha (IL-1 alpha) was inhibited by asiaticoside treatment. Asiaticoside up to 50 mutextrm{M} still required to inhibit COX-2 and IL-1 alpha induced by LPS. Consistent with these findings, treatment with asiaticoside suppressed do novo synthesis and cellular accumulation of prostaglandin E_2 to a lesser extent, suggesting that asiaticoside blocked the induction as well as the activity of COX-2 These results suggest the possibility that asiaticoside may be effective therapeutic agents for septic shock and other inflammatory diseases.
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KEYWORD
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Asiaticoside, Inducible nitric oxide synthase, Cyclooxygenase-2, Lipopolysaccharide, Anti - inflammatory activities
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